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. 2017 Aug 3;12(8):e0182143. doi: 10.1371/journal.pone.0182143

Fig 2. Mutating the tRNA-binding sites of Gcn2 abolishes activation in response to UVC irradiation.

Fig 2

A Alignment of the HisRS-like domain of budding yeast and fission yeast Gcn2. B The indicated strains were irradiated with 1100 J/m2 and samples were taken at the indicated times after irradiation. eIF2α phosphorylation was detected by immunoblotting, α-tubulin levels are shown to check even loading.