Table 2. Summary of proteins identified by tandem mass spectrometry from 2D gel.
Differential bands were excised from W50-NIDCR (highly enriched red/orange spots) and identified using mass spectrometry (MS). The spots corresponding to each protein, and the total number of peptides identified as belonging to the protein during the MS analyses, are shown in columns 3 and 4.
| Accession | Protein | Spots | Total number of Peptides |
|---|---|---|---|
| WP_005874718.1 | SusC/RagA family TonB-linked outer membrane protein | 9,14,20,23 | 37 |
| AAB49691.1 | hemagglutinin | 22 & 10 | 17 |
| P46071.1 | Protease PrtH | 10 & 22 | 17 |
| AAX47719.1 | RagA3 | 8,9,20 | 14 |
| WP_012457436.1 | T9SS C-terminal target domain-containing protein | 7 | 13 |
| XP_001386199.1 | hypothetical protein PICST_73499 | 19 | 13 |
| WP_005873522.1 | zinc carboxypeptidase | 7 | 12 |
| WP_004583425.1 | membrane protein | 12 & 14 | 11 |
| WP_012457815.1 | collagen-binding protein | 19 | 11 |
| AAX47723.1 | RagA2 | 9, 11, 20 | 11 |
| WP_012457400.1 | SusC/RagA family TonB-linked outer membrane protein | 9,10,20 | 11 |
| AAA69539.1 | Arg-gingipain-1 proteinase | 22 | 10 |
| AAC18876.1 | arginine-specific thiol protease precursor | 22 | 9 |
| WP_010956404.1 | hypothetical protein | 7 | 9 |
| XP_001010368.1 | hypothetical protein TTHERM_00354700 | 20 | 9 |
| WP_012457845.1 | hypothetical protein (old name TPR domain protein) | 16 | 9 |
| WP_012458653.1 | peptidase C25 (old name arginine-specific cysteine proteinase RgpA) | 22 | 8 |
| AAD01810.1 | hemagglutinin/protease | 22 | 8 |
| WP_004585461.1 | hypothetical protein | 16 | 8 |
| WP_010956068.1 | peptidase (old name extracellular protease) | 12 | 7 |
| P72197.1 | Lys-gingipain, catalytic subunit;39 kDa adhesin | 10 | 7 |
| Q51817.1 | Lys-gingipain W83; PrtK48; Lys-gingipain catalytic subunit | 10 | 7 |
| CAA57997.1 | protease precursor | 22 | 7 |
| AAX47715.1 | RagA4 | 12 and 9 | 7 |
| WP_012458254.1 | peptidase | 12 | 6 |
| YP_003987329.1 | putative ankyrin repeat protein | 23 | 6 |
| ZP_04009894.1 | cell wall surface anchor family | 16 | 5 |
| CAN81791.1 | hypothetical protein VITISV_020569 | 20 | 5 |
| Q51817.1 | Lys-gingipain W83; Lys-gingipain catalytic subunit;39 kDa adhesin | 22 | 5 |
| CAA68897.1 | TonB-linked adhesin | 22 | 5 |
| AAR37428.1 | hypothetical protein MBMO_EBAC750 | 20 | 4 |
| AAS68176.1 | Lys-gingipain | 22 | 4 |
| NP_721075.1 | methyltransferase | 23 | 4 |
| WP_015836135.1 | hypothetical protein | 20 | 3 |
| WP_005874011.1 | hypothetical protein | 17 | 3 |