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Absorption spectral changes of 6 μM wild-type LepHO (A) or F157I mutant (B) before (---) and after the addition of 1.2 μM LepFNR (―) in the presence of NADPH. Autoxidation of the ferrous LepHO-heme complex in air. Wild type LepHO (C) and F157I mutant (D) before (---) and after bubbling with O2 (―).
