Table 1. Selection of sample dilution.a.
| %CV | Low | MedLo | Med | High |
|---|---|---|---|---|
| ALL | 32.12% | 24.73% | 6.72% | 13.14% |
| 1:50, 1:100 | 5.88% | 4.68% | 4.76% | 4.44% |
| 1:25, 1:50, 1:100 | 8.05% | 6.08% | 5.67% | 17.41% |
| 1:50, 1:100, 1:200 | 16.39% | 10.50% | 5.08% | 5.24% |
| 1:100, 1:200, 1:400 | 26.46% | 21.63% | 2.28% | 4.31% |
a Four samples with previously characterized anti-BG-Atri IgM levels of low, medium-low, medium and high values were tested at five different dilutions (1:25, 1:50, 1:100, 1:200 and 1:400). Each result was interpolated to the standard curve, adjusted for dilution, and %CVs were calculated in comparison to all four dilutions or a subset. Two dilutions at 1:50 and 1:100, provided good agreement (after adjusting for dilution factor) across all four samples, with %CV under 6% for all samples.