Table 2. Assay specificitya.
Serum dilution | Signal:Background | Fold change | |
---|---|---|---|
−BG-A | +BG-A | ||
1:20,000 | 5.1 | 1.5 | 3.3 |
1:10,000 | 18.0 | 1.2 | 15.1 |
1:5,000 | 39.1 | 1.5 | 25.5 |
1:4,000 | 47.7 | 2.4 | 20.3 |
1:1,000 | 197.7 | 4.6 | 42.6 |
a Assay specificity. When positive serum was pre-incubated with 25mg/mL BG-A, a substantial, dose-dependent decrease in signal is observed.