Figure 1.

Three-Dimensional Model of the Anti-CD123/CD123 Complex and SPR Binding Analysis

(Top) Cartoon visualization of CD123 domain 1 (blue, with the region important for anti-CD123 binding in cyan) and anti-CD123 antibody (heavy and light chain in dark and light gray, respectively). Single mutations, shown as stick, were introduced in the antigen binding loops to affect CAR affinity. (Bottom) SPR sensogram showing the binding of immobilized WT single-chain antibody, CAM-L, and CAM-M to CD123. Raw data are shown in gray, whereas the fit used to calculate the binding properties is in color, with gradation indicating different concentrations. Association, dissociation, and binding affinity are shown.