Figure 2.

Later Effector Functions, and Not Early Cytotoxic Profile, Are Impaired in CAR-CIK Cells Displaying Identical Binding Affinity but Reduced Expression

(A and B) CAR expression (A) and MFI values (B) at day 21 of anti-CD123 CAR-CIK cells and NO DNA control. Data represented are the result of mean ± SEM. (A) n = 6 (**p < 0.01; one-way ANOVA, Bonferroni test); (B) n = 9 (**p < 0.01; ***p < 0.001; one-way ANOVA, Dunn’s multiple comparison test). (C–F) Short-term AnnV-7AAD assay by means of the cytotoxic double-target challenge at an effector-target (E:T) ratio of 5:1 after a 4 hr co-culture with both THP-1 and MHH-CALL-4 cell lines (C). Long-term proliferation assay after a 72 hr co-culture of the effector cells with THP-1 and MHH-CALL-4 cell lines (D). Intracellular cytokine staining of IFN-γ (E) and IL-2 (F) after a 5 hr co-culture between effector cells and both THP-1 and MHH-CALL-4 targets. Data represented are the result of mean ± SEM. n = 4 (***p < 0.001; two-way ANOVA, Bonferroni test).