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. 2017 May 4;25(8):1933–1945. doi: 10.1016/j.ymthe.2017.04.017

Figure 5.

Figure 5

Target Antigen Density, and Not CAR Binding Affinity, Affects Later Effector Functions

(A) Long-term proliferation assay after the co-culture of the effector cells with the indicated cell lines for 72 hr. (B–E) Intracellular cytokine staining of IFN-γ (B), TNF-α (C), IL-2 (D), and IL-6 (E). Effector cells were co-cultured for 5 hr with THP-1, KG-1, U937, TIME, and MHH-CALL-4 targets. Data represented are the result of mean ± SEM. n = 12 (ALONE condition); n = 9 (THP-1, U937, and TIME experimental cell conditions); n = 3 for all CARs tested against the KG-1 cell line (*p < 0.05; **p < 0.01; ***p < 0.001; two-way ANOVA, Bonferroni test).