Figure 2.

Knockdown of HOXD-AS1 Inhibits Prostate Cancer Cell Proliferation by Inducing G2/M Cell Cycle Arrest

(A) Efficiency of HOXD-AS1 knockdown in LNCaP, LNCaP-Bic, LNCaP-AI, and PC-3 cells by two siRNAs was verified by real-time qPCR. (B) Influence of HOXD-AS1 knockdown on viability of LNCaP and PC-3 cells in normal cultural medium measured by the MTT assay. The results are presented as the means ± SD of values obtained in three independent experiments. (C) The effect of HOXD-AS1 knockdown on LNCaP, LNCaP-Bic, and LNCaP-AI cells in androgen-deprived cultural medium. The results are presented as the means ± SD of values obtained in three independent experiments. (D) Effect of HOXD-AS1 knockdown on colony formation was measured in LNCaP and PC-3 cells. Scale bar, 600 μm. The histogram showed the mean ± SD of colonies from three independent experiments. (E) LNCaP and PC-3 cells were transfected with HOXD-AS1 siRNAs or control siRNA for 48 hr, and the cell cycles were analyzed by flow cytometry. (F) HOXD-AS1 silencing LNCaP and PC-3 cells were incubated with phospho-histone H3 antibody and analyzed by flow cytometry. Percentages (%) of cell populations at different stages of cell cycles are listed within the panels. All histograms show the percentage (%) of cell populations from three independent experiments. See also Figures S3 and S4. *p < 0.05; **p < 0.01.