Table 1. Escherichia coli K12 trains used in this study.
| Strain/Plasmid | Genotype | Source/Reference |
|---|---|---|
| CAG12080 | zah281::Tn10 | [108] |
| CH2139 | MG1655 rpsL141 | Christophe Herman |
| FC36 | Δ(lac proB)XIII thi ara RifR | [42] |
| FC40 | FC36 [F'å45 = F' proAB+ lacIq lacI33ΩlacZ] | [42] |
| PJH18 | FC40 [F’ lac-amplified] | [43] |
| PJH33 | FC40 [F’ lac-amplified] | [43] |
| PJH51 | FC40 [F’ lac-amplified] | [43] |
| PJH1390 | SMR4562 ΔrpoS::KanFRT | SMR4562xP1(SMR10336) |
| PJH1399 | SMR4562 ΔrpoS::FRT | PJH1390 x pCP20 |
| PJH1952 | SMR4562 Δdps::KanFRT | SMR4562xP1(JW0797)* |
| PJH2608 | SMR4562 Δdps::FRT | PJH1952 x pCP20 |
| PJH2947 | SMR4562 soxR104 zjc2206::Tn10Kan | SMR4562xP1(JTG1048) [31] |
| PJH2982 | JA200 [pNT3] | [60] |
| PJH3178 | SMR4562 rpsL141 | SMR4562xP1(CH2139) |
| PJH3210 | JA200 [pNT3/sodB] | [60] |
| PJH3211 | JA200[pNT3/mutM] | [60] |
| PJH3231 | FC36 ΔaraBAD567 Δzie3913.1::tetRtetA+1 FRT Δzie3920.5::3ChiKanI-SceIsite Δattλ:: PBADI-SceI dinB50::FRT [pNT3] | SMR10868 conjugated with [pNT3] |
| PJH3232 | FC36 ΔaraBAD567 Δzie3913.1::tetRtetA+1 FRT Δzie3920.5::3ChiKanISceIsite Δattλ:: PBADISceI [pNT3] | SMR10866 conjugated with [pNT3] |
| PJH3233 | FC36 ΔaraBAD567 Δzie3913.1::tetRtetA+1 FRT Δzie3920.5::3ChiKanI-SceIsite Δattλ:: PBADI-SceI [pNT3/mutM] | SMR10866 conjugated with PJH3211 |
| PJH3237 | FC36 ΔaraBAD567 Δzie3913.1::tetRtetA+1 FRTcatFRT Δattλ:: PBADI-SceI [pNT3] | SMR10798 conjugated with [pNT3] |
| PJH3240 | FC36 ΔaraBAD567 Δzie3913.1::tetRtetA+1 FRT Δzie3920.5::3ChiKanI-SceIsite Δattλ:: PBADIsceI rpoS::FRT [pNT3] | SMR10865 conjugated with [pNT3] |
| PJH3255 | FC36 ΔaraBAD567 Δzie3913.1::tetRtetA+1 FRT Δzie3920.5::3ChiKanISceIsite Δattλ:: PBADIsceI [pNT3/mutY] | SMR10866 conjugated with PJH3259 |
| PJH3256 | FC36 ΔaraBAD567 Δzie3913.1::tetRtetA+1 FRT Δzie3920.5::3ChiKanI-SceIsite Δattλ:: PBADI-SceI [pNT3/mutT] | SMR10866 conjugated with PJH3258 |
| PJH3257 | FC36 ΔaraBAD567 Δzie3913.1::tetRtetA+1 FRT Δzie3920.5::3ChiKanISceIsite Δattλ:: PBADI-sceI [pNT3/sodB] | SMR10866 conjugated with [pNT3/sodB] |
| PJH3258 | JA200 [pNT3/mutT] | [60] |
| PJH3259 | JA200 [pNT3/mutY] | [60] |
| PJH3278 | SMR4562 oxyR2 yji-eptC Kan::FRT | SMR4562xP1(SMR20958) |
| PJH3295 | FC40 oxyR2 yji-eptC Kan::FRT Δdps::FRT | PJH2608xP1(SMR20958) |
| SMR601 | ruvC53 eda51::Tn10 | [109] |
| SMR789 | FC40 ruvC53 eda51::Tn10 | FC40 x P1(SMR601) |
| SMR4562 | Independent construction of FC40 | [106] |
| SMR4610 | SMR4562 recA::Tn10dCam | [110] |
| SMR5889 | SMR4562 ΔdinB50::FRT [F' ΔdinB50::FRT] | [41] |
| SMR6906 | SMR4562 ruvC53 eda::Tn10dCam | By linear replacement [107] into SMR789 |
| SMR8847 | SMR4562 [F' zah281::Tn10 Lac+] | SMR4562 x P1(CAG12080) [108] |
| SMR10308 | SMR4562 [F' lafU2::FRTcatFRT dinB21(oc)] | [41] |
| SMR10798 | FC36 ΔaraBAD567 Δzie3913.1::tetRtetA+1 FRTcatFRT Δattλ:: PBADI-sceI | [7] |
| SMR10865 | FC36 ΔaraBAD567 Δzie3913.1::tetRtetA+1 FRT Δzie3920.5::3ChiKanISceIsite Δattλ:: PBADIsceI rpoS::FRT | [7] |
| SMR10866 | FC36 ΔaraBAD567 Δzie3913.1::tetRtetA+1 FRT Δzie3920.5::3ChiKanI-SceIsite Δattλ:: PBADIsceI | [7] |
| SMR10868 | FC36 ΔaraBAD567 Δzie3913.1::tetRtetA+1 FRT Δzie3920.5::3ChiKanI-SceIsite Δattλ:: PBADI-SceI dinB50::FRT | [7] |
| SMR12566 | SMR4562 ΔrssB::tet | [75] |
| SMR15378 | SMR4562 Δattλ:: PBADmutLcat | By replacement of λ prophage [111] in SMR8166 |
| SMR20958 | MG1655 Δattλ::PsulAΩsRBS75mCherry FRT IN-FOG84 | [112] |
*All JW strains are from the Keio Collection, described in [113].