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. 2017 Jul 24;13(7):e1006518. doi: 10.1371/journal.ppat.1006518

Fig 6. Effect of O-GlcNAcylation on the HTLV-1 LTR.

Fig 6

(A) Effect of Thiamet G on Ser133 phospho-CREB recruitment to the vCRE LTR region. C8166 T cells were cultured with or without Thiamet G for 48h before chromatin preparation. Chromatin was precipitated with either control IgG or an anti-Ser133 phospho-CREB and recovered DNA was amplified using a pair of primers specific for the vCRE sequence. Results correspond to means ± SEM of triplicate determinations obtained in a representative experiment out of 2. (B-D) Detection of OGA or OGT on the vCRE sequence by ChIP in C8166 (B) and MT2 (C) HTLV-1-transformed T cells or in HTLV-1-immortalized CIB T cells (D). Cells were treated as above and chromatin was precipitated using anti-OGT, anti-OGA or control (IgG) antibody. Recovered DNA was amplified using pairs of primers specific for the vCRE sequence or for alpha-satellite sequences as negative control. Results correspond to means ± SEM of triplicate determinations obtained in a representative experiment out of 2.