Table 1. Primers and probes used for real-time quantitative and standard PCR in this study.
| Microorganisms | Targeted sequence | Primers f, r (5’-3’) and probes p (6FAM-TAMRA) | References |
|---|---|---|---|
| qPCR primers | |||
| Rickettsia spp. | gltA(RKND03) | f_GTGAATGAAAGATTACACTATTTAT | [72] |
| r_GTATCTTAGCAATCATTCTAATAGC | |||
| p_CTATTATGCTTGCGGCTGTCGGTTC | |||
| R. africae | poT15-dam2 | f_TGCAACACGAAGCACAAAAC | [32] |
| r_CCTCTTGCGAAACTCTACTT | |||
| p_TGACGTGTGGATTCGAGCACCGGA | |||
| Anaplasma spp. | 23SrRNA (TtAna) | f_TGACAGCGTACCTTTTGCAT | [34] |
| r_TGGAGGACCGAACCTGTTAC | |||
| p_GGATTAGACCCGAAACCAAG | |||
| Borrelia spp. | (Bor ITS4) | f_GGCTTCGGGTCTACCACATCTA | [62] |
| r_CCGGGAGGGGAGTGAAATAG | |||
| p_TGCAAAAGGCACGCCATCACC | |||
| (Bor_16S) | f_AGCCTTTAAAGCTTCGCTTGTAG | [73] | |
| r_GCCTCCCGTAGGAGTCTGG | |||
| p_CCGGCCTGAGAGGGTGAACGG | |||
| C. burnetii | (IS30A) | f_CGCTGACCTACAGAAATATGTCC | [74] |
| r_GGGGTAAGTAAATAATACCTTCTGG | |||
| p_CATGAAGCGATTTATCAATACGTGTATG | |||
| Bartonella spp. | (Barto ITS2) | f_GATGCCGGGGAAGGTTTTC | [75] |
| r_GCCTGGGAGGACTTGAACCT | |||
| p_GCGCGCGCTTGATAAGCGTG | |||
| Standard PCR primers | |||
| Rickettsia spp. | gltA | f_ATGACCAATGAAAATAATAAT | [33] |
| r_CTTATACTCTCTATGTACA | |||
| Anaplasma spp. | 23SrRNA | f_ATAAGCTGCGGGGAATTGT | [34] |
| r_TGCAAAAGGTACGCTGTCAC | |||
| Borrelia spp. | flaB | f_TGGTATGGGAGTTTCTGG | [35] |
| r_ TAAGCTGACTAATACTAATTACCC |