Table 4. Tick species selected to create a MALDI-TOF MS reference database, identified by molecular biology.
| Morphological identification | Origin | Number of specimens tested | Intra-species similarity of 12S rRNA gene sequence (%) | Molecular identification by BLAST (Accession Number) | Query cover (%) | Similarity level with GenBank (%) |
|---|---|---|---|---|---|---|
| Am. variegatum | Bamako, Kollé, Bougoula | 5 | 100% | Am. variegatum (JF949801.1) | 100% | 100% |
| Hy. truncatum | Bamako, Kollé, Bougoula | 5 | 99–100% | Hy. truncatum (AF150031.1) | 99–100% | 96–97% |
| Hy. m. rufipes | Bamako, Kollé, Bougoula | 3 | 100% | Hy. m. rufipes (KC817342.1) | 100% | 100% |
| Rh(Bo) microplus | Kollé, Bougoula | 3 | 99–100% | Rh(Bo) microplus (DQ003008.1) | 100% | 99–100% |
| Rh. e. evertsi | Bamako | 2 | 100% | Rh. e. evertsi (KU255856.1) | 99% | 100% |
| Rh. sanguineus sl | Bougoula | 2 | 100% | Rh. sanguineus (KC817342.1) | 100% | 100% |
| Rh(Bo) microplus* | Kollé, Bougoula, Bamako | 26 | 100% | Rh. microplus (KY020993.1) | 100% | 100% |
*Ticks identified by 16S rRNA gene