Table 2. Validation of the transcriptional up-regulation of five candidate genes through qRT-PCR.
| qRT-PCR | Microarray | ||
|---|---|---|---|
| Transcript | Reference strain | Fold change (95% CI) | Fold Change |
| Hexamerin (AAEL013757) | Rockefeller | 63 (36–90) | 57 |
| New Orleans | 123 (54–191) | 97 | |
| Cyp9J32 (AAEL008846) | Rockefeller | 17 (13–21) | 41 |
| New Orleans | 107 (81–132) | 53 | |
| Cuticular (AAEL002246) | Rockefeller | 42 (16–69) | 36 |
| New Orleans | 65 (25–105) | 51 | |
| Cyp9J28 (AAEL014617) | Rockefeller | 13 (6–21) | 31 |
| New Orleans | 104 (63–144) | 30 | |
| GSTd4 (AAEL001054) | Rockefeller | 169 (34–305) | 24 |
| New Orleans | 268 (148–387) | 22 |
The relative expression ratio of five candidate genes in the Funchal population compared to two susceptible laboratory colonies (New Orleans and Rockefeller) is shown. Values are estimated from four biological replicates and 95% confidence intervals are shown. Estimated values from the microarray experiment are given for comparison.