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. 2017 Jul 24;13(7):e1006531. doi: 10.1371/journal.ppat.1006531

Fig 4. Vag8 inhibits complement-mediated killing of bacteria.

Fig 4

(A) Complement-mediated killing of luminescent E. coli in 1.25% NHS is inhibited in the presence of Vag8 but not Prn. (B) No inhibitory effect on complement-mediated killing was shown for Vag8 in 5% NHS. (C) Vag8 inhibits complement-mediated killing of luminescent E. coli in 5% fDd serum. (D) B1917 wild type OMVs containing Vag8 inhibit complement-mediated killing of E. coli using traditional serum killing and CFU counting, as well as of (E) luminescent E. coli. (F) Complement-mediated killing of luminescent E. coli is inhibited using 7.5 μg/ml or more Vag8. Data shown in A-C and E-F is a representative figure of three separate experiments. Data shown in D represent the mean ± SEM of three separate experiments. ** p≤0.01, ***≤0.001