Table 1. Characteristics of strains used in this study.
| Bacteria | Strain | Plasmid or deletion | Antibiotica | Reference |
|---|---|---|---|---|
| B. pertussis | B1917 | Streptomycin-resistant derivative | This study | |
| B1917 | ΔVag8 | KAN 50 μg/ml | This study | |
| E. coli | Top10f | pGEM Vag8::KAN | KAN 50 μg/ml/ AMP 100 μg/ml | This study |
| SM10 | KAN 50 μg/ml | [62] | ||
| Top10F | pSS1129(pertussis cloning vector) | Amp 100 μg/ml / Gm 10 μg/ml | [62] | |
| DH5a | NM522 (kan cassette) | KAN 50 μg/ml | [63] | |
| MG1655 | WT | [64] | ||
| MG1655 | pUC18-mini-Tn7T-Gm-lux | Amp 100 μg/ml | [64] | |
| Top10F1 | pRSETb-Vag8 | Amp 100 μg/ml | This study | |
| BL21 | pRSETb-Vag8 | Amp 100 μg/ml | This study | |
| Top10F | pGEM-T::Vag8 | Amp 100 μg/ml | This study | |
| Top10F | pGEM-T::Vag8::KAN | Amp 100 μg/ml/ 50 μg/ml | This study | |
| Top10F | pSS1299::Vag8::KAN | Amp 100 μg/ml/ 50 μg/ml | This study |