Table 1.

Primers used in PCR studies

Gene	Primer 5′–3′	Tm (°C)	Product (bp)	Acc. number
ratGapdh	F: GCA AGT TCA ACG GCA CAG TCA AGG	61.6	116	NM_017008
	R: AGC ACC AGC ATC ACC CCA TTT G	60.5
ratMMP‐2	F: CTG ACA TCA TGA TCA ACT TTG GAC G	55.9	322	NM_031054
	R: GCA GAA GCC ATA CTT GCC ATC C	56.6
ratMMP‐9	F: ACC GCC AAC TAT GAC CAG GAT AAG C	60.4	110	NM_031055
	R: AAG ACG AAG GGG AAG ACG CAC ATC	61.4
ratTIMP3	F: ACT TGC CTT GCT TTG TGA CCT CC	58.4	166	NM_012886
	R: TTG CTG ATG CTC TTG TCT GGG G	57.6
ratTIMP4	F: GAG AGC CTG AAT CAT CAC TAC CAC C	57.0	106	NM_001109393
	R: CAG TCA GTC CAG AGA CAC TCA TTG	57.0
ratCx40	F: GGC TCA CCG TCC TGT TCA TTT TC	58.5	128	NM_019280
	R: GGT CGT AGC AGA CAT TTT GGC AAC	58.7
ratCx43	F: TCC TTG GTG TCT CTC GCT TTG AAC	58.5	130	NM_012567
	R: AGT CTT TTG ATG GGC TCA GTG GG	58.5
ratCx45	F: AGA GCA GAG CCA ACC AAA ACC C	58.3	114	NM_001085381
	R: AAG CCC ACC TCA AAC ACA GTC CTG	60.2
ratCol type 1	F: TGG AGA AGA AGG AAA ACG AGG AGC	59.0	115	NM_053304
	R: AAC ACC ATC AGC ACC AGG GAA AC	58.8
ratCol type 3	F: TCC TGG TGC TAT TGG TCC ATC TGG	60.8	164	NM_032085
	R: TGC GTC CAT CAA AGC CTC TGT GTC	62.1
ratTGF‐β	F: TCA AGT CAA CTG TGG AGC AAC ACG	58.8	177	NM_021578
	R: AGC GAA AGC CCT GTA TTC CGT C	56.8
ratIL‐6	F: ACT TCC AGC CAG TTG CCT TCT TG	58.5	107	NM_012589
	R: TCT GTT GTG GGT GGT ATC CTC TGT G	58.8

Acc, accession; Col, collagen; Cx, connexin; Gapdh, glycerinaldehyde‐3‐phosphate dehydrogenase; IL, interleukin; MMP, matrix metalloproteinase; TGF, transforming growth factor; TIMP, tissue inhibitors of metalloproteinase; Tm, melting temperature.