Figure 3.

Experimental Setup and Presence of hSEAP and hFIX Vector DNA and mRNA in the Liver

(A) Monkeys were injected i.v. at day 0 with an AAV5^ch-based vector (AAV5^ch-hSEAP) or PBS and at day 30 with either an AAV1- or AAV5^ch-based vector (AAV1-hFIX or AAV5^ch-hFIX). (B–E) Reporter gene hSEAP DNA (B), mRNA (C), and hFIX DNA (D) and mRNA (E) in the monkey liver tissue at sacrifice. Mean ± SEM of eight different liver regions was plotted for every monkey. Monkeys were injected i.v. at day 0 with an AAV5^ch-hSEAP (HNPs 10–15) or nothing (n. 1, n. 2) and at day 30 with AAV1-hFIX (HNPs 4–6 and 10–13) or AAV5^ch-hFIX (HNPs 14 and 15) and sacrificed at day 364.