Table 2.

Cβ-Cβ and N-Cβ interresidue distance restraints, measured in the molecular models of the CRF- and ^dFXCRF(12-41)-CRF1R complexes.

DOI: http://dx.doi.org/10.7554/eLife.27711.012

CRF1R bound to agonist CRF
No	CRF-CRF1R residue pair	Region of CRF	Region of CRF1R	Cβ-Cβ distance [Å]
				Constraint	Measured
1	H13-F330	N-term	Helix VI	10.0	5.1
2	H13-F344	N-term	Helix VII	10.0	9.4
3	L15-Q173	N-term	Helix II	10.0	7.1
4	E17-I345	N-term	Helix VII	10.0	9.2
5	V18-V120	C-term	Helix I	10.0	6.4
6	A31-E104	C-term	Hinge	10.0	8.6
7	S33-Y73	C-term	ECD	10.0	6.7
CRF1R bound to Antagonist dFXCRF(12-41)
No	CRF1R-dFXCRF(12-41) residue pair	Region of dFXCRF(12-41)	Region of CRF1R	Cβ-Cβ or N-Cβ* distance [Å]
				Constraint	Measured
1	F12-L329	N-term	Helix VI	5.0*	4.4*
2	F12-F330	N-term	Helix VI	5.0*	4.6*
3	F12-N348	N-term	Helix VII	5.0*	4.8*
4	H13-L329	N-term	Helix VI	10.0	6.7
5	H13-F330	N-term	Helix VI	10.0	5.5
6	H13-F344	N-term	Helix	10.0	5.0
7	L14-N123	N-term	Helix I	10.0	9.1
8	L15-R341	N-term	Helix VII	10.0	5.9
9	L15-I345	N-term	Helix VII	10.0	6.8
10	E17-N123	N-term	Helix I	10.0	7.4
11	E17-Q173	N-term	Helix II	10.0	6.3
12	E17-I345	N-term	Helix VII	10.0	6.1
13	V18-V120	C-term	Helix I	10.0	4.7
14	A31-E104	C-term	Hinge	10.0	4.3
15	S33-Y73	C-term	ECD	10.0	6.7

^*Pair-wise crosslinking between N-terminal ClAc in the peptide and Cys thiol in CRF1R.