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. 2017 Aug 3;121(4):354–367. doi: 10.1161/CIRCRESAHA.116.310529

Figure 4.

Figure 4.

Diet- and age-dependent splenomegaly in double knockout (DKO) mice. A, Spleen weight in Ldlr−/− or DKO mice after 12 or 18 wk of high cholesterol diet (HCD; n=12–16 for 12-wk HCD; n=5 for 18-wk HCD). B, Representative images of spleens and femurs in wild-type (WT) and KO bone marrow transplant (BMT) mice on HCD for 12 wk. C, Quantification of spleen weight in WT and KO BMT mice on HCD for 12 wk (n=5–7). D, Mice were placed on HCD or normal chow diet (NCD) at 20 wk of age for 12 wk. E, Percentage of Oil Red-O (ORO) region per aortic arch measured en face (n=3–4). Ldlr−/− mice on NCD were from a separate experiment and are included for comparison purposes (n=5). F, Representative images of spleens and femurs. G, Quantification of spleen weights (n=3–4). Ldlr−/− mice on NCD were from a separate experiment and are included for comparison purposes (n=5). H, Quantification of total CD45+ cells in spleens by fluorescence-activated cell sorting (FACS) analysis (n=3–4). Ldlr indicates low-density lipoprotein receptor.