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. 2017 Aug 3;121(4):354–367. doi: 10.1161/CIRCRESAHA.116.310529

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© 2017 The Authors.

Circulation Research is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

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Figure 6. — MicroRNA-146a (miR-146a) in bone marrow (BM)–derived cells regulates BM and extramedullary hematopoiesis and levels of circulating leukocytes and lymphocytes. Lethally irradiated Ldlr^−/− mice (mix of males and females) were reconstituted with BM from wild-type (WT BM) or miR-146a^−/− (knockout [KO] BM) donors, followed by high cholesterol diet (HCD) for 12 wk. Increase of splenic (A) and a decrease of BM (B) leukocytes and lymphocytes, as assessed by fluorescence-activated cell sorting (FACS) analysis (n=5–7). C, Western blot of TRAF6 (TNF receptor–associated factor 6) and phospho-p65 (normalized to β-actin and total p65, respectively), in mice receiving WT or KO BM after 12 wk of HCD. Phospho-p65/p65 blots from WT/KO animals are from the same membrane with identical imaging parameters. D, Decrease of a subset of multipotent stem cells, but no changes to the long-term HSCs in the BM of mice receiving KO BM transplants (BMT; n=5–7). E, Increase of splenic hematopoietic and multipotent stem cells in mice receiving KO BM (n=5–7). F, Early monocytosis (4-wk HCD), followed by a decrease in peripheral blood (PB) proatherogenic cells (neutrophils, B Cells, and Ly6C^hi monocytes), and an increase of antiatherogenic Ly6C^lo monocytes after 12 wk of HCD (n=3 for 4-wk HCD; n=9–11 for 12-wk HCD). CMP indicates common myeloid progenitor; GMP, granulocyte–macrophage progenitor; HPC, hematopoietic progenitors cell; HSC, hematopoietic stem cell; Ldlr, low-density lipoprotein receptor; LS⁻K, Sca-1 (stem cells antigen-1)–negative progenitor; MEP, megakaryocyte–erythroid progenitor; and MPP, multipotent progenitor cell.