Figure 3.

Western Blot Analysis of RPGR Expression and Glutamylation

(A) RPGR^ORF15 expression (black arrowhead) was detected in HEK293T cells transfected with either coRPGR^ORF15 (co) or wtRPGR^ORF15 (wt) containing plasmids compared with untransfected samples (UNT). A truncated 80 kDa protein (white arrowhead) was detected with an N terminus-directed RPGR antibody in cells transfected with the WT plasmid compared with cells transfected with the codon-optimized plasmid. (B) Glutamylated RPGR^ORF15 was detected with the GT335 antibody in HEK293T cells transfected with the codon-optimized and the WT sequence of RPGR^ORF15. (A and B) The 80 kDa band in (A) was not glutamylated in (B) and may therefore represent a truncated RPGR variant with a C-terminal deletion.