Figure 1.

Recombinant AAV Vectors Used in the Study

(A and B) rAAV vectors containing a human IRBP promoter (IRBP Pro) or human GRK1 promoter (GRK1 Pro) driving expression of a humanized enhanced GFP reporter gene (eGFP). (C–F) rAAV vectors containing a human GRK1 promoter or human IRBP promoter driving expression of different versions of the human RPGR cDNA. hRPGRstb and hRPGRco are two versions of human RPGR^exon1-ORF15, with nucleotide sequence differences in exon ORF15 described in Figure S5. With the exception of bGH pA not being included in constructs (D) and (E), all other elements (SV40 SD/SA and poly A signals) within these constructs are identical. TR, AAV2-inverted terminal repeats; SV40 SD/SA, simian virus 40 splice donor/splice acceptor element; SV40 pA, simian virus 40 polyadenylation signal; bGH pA, bovine growth hormone polyadenylation signal.