Figure 6.

Constitutive AMPK Activation and Increased Mitochondrial Activity in the Livers of Kmt5a^ΔHepA Mice

(A) ATP and ADP levels in liver extracts from P45 wild-type and Kmt5a^ΔHepA mice. n = 5. ^∗∗p < 0.01; ^∗∗∗p < 0.001.

(B) Western blot analysis of liver extracts with antibodies recognizing a Thr172-phosphorylated form of AMPK (top panel) or total AMPK protein (bottom panel).

(C) Representative immunohistological staining of liver sections with anti-carbamoyl phosphate synthetase (CPS) antibody recognizing the mitochondrial CPS1 enzyme. Panels at the right show cytochrome oxidase I (COI) and cytochrome oxidase IV (COIV) enzymatic activities in liver extracts from P45 wild-type and Kmt5a^ΔHepA mice. n = 5. ^∗∗p < 0.01; ^∗∗∗p < 0.001.

(D) Electron microscopic images of liver sections from P45 wild-type and Kmt5a^ΔHepA mice. Blue arrows indicate normal-sized mitochondria. Red arrows indicate enlarged mitochondria. Green arrows depict autophagosome structures. Note the larger magnification in the bottom-right panel.

(E) Western blot analysis of liver extracts with an antibody recognizing the autophagy marker protein LC3 (top panel) and Gapdh (bottom panel).

See also Figure S6.