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Chronic VEGF infusion does not lead to a reduction in claudin-5 expression in the juxtacanalicular region. Claudin-5 expression was not reduced in the juxtacanalicular region with VEGF infusion unlike as reported in previous studies with a single-dose VEGF injection. Robust cytoplasmic claudin expression was noted in regions of neovascularization. In uninfused brain (Opp), claudin-5 was localized to a membranous pattern as expected. Bar = 100 μm.
