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VEGF leads to deranged neovascularization. Although AQP4 overexpression is noted in juxtacanalicular regions with VEGF infusion, inadequate coverage (arrowheads) of newly formed endothelial tubes (CD105 [FITC]) is observed (lower row). In uninfused brain (upper row), AQP4 tubes (Texas red) are closely aligned with endothelial tubes (CD105 [FITC]) demonstrated by colocalization on the merged image. Bar = 100 μm.
