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. 2017 Feb 17;21(8):1532–1544. doi: 10.1111/jcmm.13084

Figure 5.

Figure 5

DDA1 promotes cell proliferation through the regulation of cyclins. (A) A549 and H1299 cells were transfected as indicated and cultured for 48 hrs. All treatments were performed for three duplications. Whole‐cell lysates were used to assess DDA1, CCND1, CCND3, CCNE1, CCNB1 and GAPDH (as a loading control) expression by Western blot analysis. (B) Whole tissue lysates from three independent tumours of DDA1‐deficient xenograft tumours from nude mice were used to evaluate DDA1, CCND1, CCND3, CCNE1, CCNB1 and GAPDH (as a loading control) expression by Western blot analysis. (C, D) Immunohistochemical probing for DDA1, PCNA, CCND1, CCND3, CCNE1 and CCNB1 using paraffin sections of DDA1‐deficient xenograft tumours from nude mice. Positive cells were counted by Image‐Pro Plus and analysed by GraphPad Prism 6. (n = 3, **P < 0.01, ***P < 0.001, Student's t‐test).