Table 1.
Target gene | Primer sequence |
---|---|
MiR‐138 | F: 5′‐GGTGTCCGTGGAGTCGGCAA‐3′ |
R: 5′‐AACTTCACAACACCAGCTTA‐3′ | |
PI3K | F: 5′‐CTTGCCTCCATTCACCACCTCT‐3′ |
R: 5′‐GCCTCTAATCTTCTCCCTCTCCTTC‐3′ | |
Akt | F: 5′‐TGTCTCGTGAGCGCGTGTTTT‐3′ |
R: 5′‐CCGTTATCTTGATGTGCCCGTC‐3′ | |
eNOS | F: 5′‐CCAGCTAGCCAAAGTCACCAT‐3′ |
R: 5′‐GTCTCG GAGCCATACAGGATT‐3′ | |
β‐actin | F: 5′‐CACCACACCTTCTACAATGAGC‐3′ |
R: 5′‐GTGATCTCCTTCTGCATCCTGT‐3′ |
Note: miR‐138, microRNA‐138; PI3K, phosphatidylinositol 3‐kinase; Akt, protein kinase B; eNOS, endothelial nitric oxide synthase.