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. 2017 Mar 31;21(8):1482–1491. doi: 10.1111/jcmm.13074

Table 1.

The primer sequences used for quantitative real‐time polymerase chain reaction

Target gene Primer sequence
MiR‐138 F: 5′‐GGTGTCCGTGGAGTCGGCAA‐3′
R: 5′‐AACTTCACAACACCAGCTTA‐3′
PI3K F: 5′‐CTTGCCTCCATTCACCACCTCT‐3′
R: 5′‐GCCTCTAATCTTCTCCCTCTCCTTC‐3′
Akt F: 5′‐TGTCTCGTGAGCGCGTGTTTT‐3′
R: 5′‐CCGTTATCTTGATGTGCCCGTC‐3′
eNOS F: 5′‐CCAGCTAGCCAAAGTCACCAT‐3′
R: 5′‐GTCTCG GAGCCATACAGGATT‐3′
β‐actin F: 5′‐CACCACACCTTCTACAATGAGC‐3′
R: 5′‐GTGATCTCCTTCTGCATCCTGT‐3′

Note: miR‐138, microRNA‐138; PI3K, phosphatidylinositol 3‐kinase; Akt, protein kinase B; eNOS, endothelial nitric oxide synthase.