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. 2017 Feb 28;21(8):1636–1647. doi: 10.1111/jcmm.13092

Figure 4.

Figure 4

Effect of ND on gene expression levels of BTB components. qRT‐PCR evaluation of Junctional adhesion molecule A (JAM1), Dynamin‐2 (DNM2), Occludin (OCLN), Gap junction alpha‐1 (GJA1) and TJP1 gene expression after ND administration and/or endurance training. The graphs show normalization with the reference genes, according to the Livak method (2−∆∆CT). Vertical axis: 2−∆∆CT. Horizontal axis: mice groups. Normal control mice (SED), sedentary low dose of ND (SEDND‐L), sedentary medium dose of ND (SED‐ND‐M), sedentary high dose of ND (SED‐ND‐H), trained control mice (TR), trained low dose of ND (TR‐ND‐L), trained medium dose of ND (TRND‐M) and trained high dose of ND (TR‐ND‐H). Data are presented as the mean ± S.D. *vs SED, TR, TR‐ND‐L and SED‐ND‐M (P < 0.05); #vs SED, TR, TR‐ND‐L and SED‐ND‐H (P < 0.01); $vs SED (P < 0.05); &vs SED, TR, TR‐ND‐L, TR‐ND‐M and SED‐ND‐H (P < 0.01); †vs SED and SED‐ND‐M (P < 0.05); ¥vs SED and SED‐ND‐H (P < 0.05); Δvs SED‐ND‐M, SED‐ND‐H, TR‐ND‐H (P < 0.05); ◊vs TR and TR‐ND‐L (P < 0.05).