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. 2017 Jun;13(2):84–92.

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© Kazuya Toda et al. Licensee Master Publishing Group

This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Effects of constituents in PFE on Per2 mRNA expression in NIH3T3 cells. After the treatment with serum-rich medium, NIH3T3 cells were cultured with or without PFE or 1-5 at 10 or 30 μg/mL for 20 h. The mRNA expression of Per2 in NIH3T3 cells was evaluated by real-time RTPCR. The mRNA expression of β-actin was used in order to correct the expression of each mRNA. Each column represents the mean and SE (n=4). Asterisks denote significant differences from the control at *p<0.05 and **p<0.01.