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. 2017 Aug 4;7:356. doi: 10.3389/fcimb.2017.00356

Figure 1.

Figure 1

Human DDX25 is ubiquitously expressed and upregulated after DENV infection. (A) qRT-PCR and electrophoresis analysis of DDX25 expression in various human tissues. Human β-actin was used as an internal control. (B) The qRT-PCR and immunoblots analysis of DDX25 expression in HEK293T, A549, and K562 cells before and after DENV infection. (C) Microscopic images of immunofluorescence staining for expression and subcellular distribution of DDX25 in HEK293T with or without DENV infection. (MOI of 1 for 24 h). Scale bars, 10 μm. (D) Immunoblots analysis of DDX25 expression in the cytoplasm and nucleus before and after DENV infection. The relative gray density of immunoblots bands (DDX25/β-actin) was analyzed by ImageJ software. Results were expressed as the mean + the SEM. *p < 0.05 and **p < 0.01 (t-test). Representative results from at least three independent experiments.