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. 2017 Aug 4;7:357. doi: 10.3389/fcimb.2017.00357

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Copyright © 2017 Yi, Huang, Guo, Zhou, Chang and Huan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GSK-3beta signaling is involved in LPS-induced HPMECs barrier disruption. The HPMECs were plated on the gold microelectrodes. When HPMECs formed monolayers and reached stable TER values, the SB-216763 (20 μM) was added. After 1 h, the medium or LPS (0.1 μg/ml) was added for another 6 h. The HPMEC monolayers permeability was determined by real-time TER measurement (A). The results of the 3 h LPS stimulation were represented as a histogram in (B) according to the TER curves. ^*P < 0.05 vs. negative control. ^#P < 0.05 vs. corresponding LPS-stimulated group.