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. 2017 Aug 3;7:7207. doi: 10.1038/s41598-017-07455-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Different culture methods affect mouth-form phenotypic plasticity. (A) Diagram of experimental design to compare culture conditions from the same population after bleach synchronization. (B) Mouth-form ratios presented as percent eurystomatous (% Eu) from the parental generation (P) and the next generation (G1) grown in either liquid culture or agar plates, n = 18 biological replicates, p < 0.05, students two-tailed t-test, error bars represent SEM. (C) Developmental stages of bleach-synchronized P. pacificus in either agar plates or liquid culture. Bar graphs represent a typical experiment measuring >30 animals at the indicated time-points. (D) Mouth-form ratios of switching experiments between agar and liquid cultures. Nematodes were bleached between generations (P, G1, G2), and eggs-J1 larvae were passed to the next condition, n = 3, error bars represent SEM.