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. 2017 Aug 3;7:7207. doi: 10.1038/s41598-017-07455-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Macro-evolutionary view of liquid culture environmental influence. (A) Phylogeny of Pristionchus species⁷⁰ highlighting P. pacificus (bold), P. fissidentatus, P. mayeri, and P. entomophagus (blue). (B) Mouth-form ratio of parental generations (n = 3) of indicated species on NGM-agar after three consecutive healthy generations on OP50. (C) Mouth-form ratios of indicated species in either NGM-agar or liquid culture/S-medium (n = 3), error bars represent SEM.