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. 2017 Aug 3;7:7240. doi: 10.1038/s41598-017-07655-1

Figure 6.

Figure 6

MEF2D regulates DYRK1A kinase activity exemplified by NFATc2 protein expression. (A) MEF2D increased DYRK1A protein and decreased NFATc2. MEF2D expression plasmid and empty vector were transfected into T98G cells with NFATc2 expression vector. NFATc2 was detected with anti-HA antibody. MEF2D was detected with anti-MEF2D antibody (610774, BD, San Jose, CA, USA). DYRK1A isoform 5 was detected with anti-DYRK1A antibody (ab156818, Abcam, Shanghai, China). β-actin detected by β-actin monoclonal antibody (SAB1403520; Sigma-Aldrich, Saint Louis, USA) was used as loading control. (B) Quantification of A. Values represent means ± SEM; n = 3; *P < 0.01 by Student’s t test. (C) DYRK1A decreased NFATc2 protein level. DYRK1A expression plasmid and empty vector were transfected into T98G cells with NFATc2 expression vector. NFATc2 was detected with anti-NFATc2 monoclonal antibody (MA1–025, ThermoFisher, Waltham, USA). DYRK1A was detected with anti-DYRK1A antibody (ab156818, Abcam, Shanghai, China). β-actin detected by β-actin monoclonal antibody (SAB1403520; Sigma-Aldrich, Saint Louis, USA) was used as loading control. (D) Quantification of C. Values represent means ± SEM; n = 3; *P < 0.01 by Student’s t test.