Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 3;7:7190. doi: 10.1038/s41598-017-05685-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Correlation between HIF-α, FASN Protein Levels and HIF Target Gene Expression in Adipogenesis and Prostate Cancer. (A) Downregulation of HIF-α protein levels by 25-OH treatment. HCT116, HeLa and 293 T cells were treated with 25-OH for 72 h and incubated for the last 4 h in 1% oxygen. HIF-α levels were analyzed by western blotting. (B) Hif-α activation during differentiation of 3T3-L1 cells into adipocytes. Confluent 3T3-L1 cells were cultured for 14 days in regular medium (1), or differentiation medium in the presence of insulin alone (2) or with insulin, dexamethasone, and 3-isobutyl-1-methylxanthine (3). Fasn, Hif-α and Redd1 expression was analyzed by western blotting. Lipid accumulation was monitored by staining with Oil-Red O. (C) Time-dependent changes of Hif target gene expression during 3T3-L1 differentiation. Inducers of differentiation were added at Day 2. mRNAs of the Hif target genes were measured by qPCR in biological triplicates and technical duplicates as a total of 6 samples and normalized against the reference gene Tbp and Day 2 samples. Data plots with p-value larger than 0.01 were indicated by red brackets and a red arrow additionally showing Vegfα at Day 2. Lipid staining results with Oil-Red O are shown under the graph for each time point. (D) Stability of Hif-1α protein. Stability was measured after oxygen concentration shift (1.0% to 21% oxygen) for undifferentiated cells, cycloheximide (CHX) addition for undifferentiated cells in hypoxia, or cycloheximide addition for differentiated cells in normoxia. (E) Gene expression profiles of prostate cancer patients obtained from IST Online (MediSapiens) was analyzed, and mRNA levels of several HIF target genes were examined in correlation with FASN levels. Patient samples co-overexpressed with FASN are indicated with a horizontal bar. M stage: metastasis, PSA: prostate-specific antigen. Experiments were repeated twice (A,B,D), and the results were reproduced. The qPCR experiment (C) was performed once with biological triplicates.