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. 2017 Aug 3;7:7184. doi: 10.1038/s41598-017-07340-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PMN-recruitment in Adamt13 ^−/− mice is impaired after infection with A. fumigatus. Wildtype (129/Sv/Pas) or Adamts13 ^−/− mice were infected i.t. with A. fumigatus (10⁷ conidia per animal) or H₂O (sham control). Peripheral blood and BAL fluid were collected 24 h after infection and analyzed by flow cytometry. (A,B) The frequency of PMN (CD11b^high Gr-1⁺) in the peripheral blood (A) and total PMN counts in the BAL fluid (B) are shown. Depicted are the cumulative results of three (H₂O-treated 129/Sv/Pas, n = 7), one (H₂O-treated Adamts13 ^−/−, n = 3) or four independent experiments (A. fumigatus-treated 129/Sv/Pas, n = 17; Adamts13 ^−/−, n = 16) plus SD. (C) For analysis of PMN phenotype in blood or BAL fluid, CD62L shedding and CD11b regulation was quantified gating on Gr-1⁺ cells. The percentage of activated PMN (Gr-1⁺ CD11b^high CD62L^low cells) of a representative sample is shown. (*) indicates a significant difference (p < 0.05) by one-way ANOVA with Bonferroni’s posttest. (D) Different cytokines and chemokines in the BAL fluid of infected mice were quantified by Bioplex-Assay. The cumulative results of one experiment (n = 3) out of two are shown. Mann-Whitney U-test calculated no significant differences between knockout and wildtype animals.