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. 2017 Aug 3;7:7184. doi: 10.1038/s41598-017-07340-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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A. fumigatus activated serum from Adamts13 ^−/− mice does not alter PMN migration. Serum from wildtype (129/Sv/Pas) or Adamts13 ^−/− mice was activated with 10⁸/ml A. fumigatus conidia (A). fumigatus activated serum; AAS) and analyzed for chemotactic ability. For transwell migration of human PMN from healthy donors towards AAS or non-activated mouse serum (NMS), serum was added to the lower chambers and calcein labeled PMN (2 × 10⁵ cells) in upper wells and incubated for 1 h at 37 °C. The cumulative results of six independent experiments are depicted. By two-tailed Wilcoxon signed rank test significant increase of migration is shown for A. fumigatus-activated serum compared to native serum in both mice types. Statistical significant differences of PMN migration towards activated serum between Adamts13 ^−/− mice compared to wildtype sera were not observed.