Figure 3.
EKP induces abnormal electrographic activities in the optic tectum and c-fos expression in brain of 7 dpf larvae. (A) Fragments of representative recordings from 7 dpf larva treated with VHC (A1) or 15 min after application EKP (400 µM) (A2). Bottom trace (A3) shows a high-resolution magnification of the epileptiform events indicated above. (B) Number of epileptiform events in VHC and EKP treated larvae: VHC 0.4857 ± 0.1804 vs EKP 55.76 ± 6.872 events/10 min recording. The number of recordings analyzed were: VHC (n = 35) and EKP (n = 58). Statistical analysis was performed using Student’s unpaired t-test. Values that were significantly different compared to VHC were indicated with ****p ≤ 0.0001. Error bars on all graphs represent s.d. (C) EKP-induced seizure upregulated c-fos expression in 7 dpf zebrafish larvae as assessed by qPCR (PTZ was used as positive control). c-fos expression is normalized to the housekeeping gene ef1α and β-actin 1. The data were analyzed using the ΔΔCq method and reported as mean ± s.e.m. of three experiments performed in triplicate.