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. 2017 Aug 3;7:7219. doi: 10.1038/s41598-017-07575-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TrxR1 overexpression delays C2C12 differentiation. C2C12 were transfected or not (mock) with p-TrxR1 or the empty vector pcDNA3.1 and analysed at 48 (D2) and 72 hours (D3) from differentiation induction. (a) Representative image of the western blot analysis showing the protein expression of TrxR1, Myogenin, MyHC and β-actin. The amount of TrxR1 (b), Myogenin (c) and MyHC (d) in each sample was calculated by normalizing to β-actin protein levels. Results are represented as mean ± SEM of at least three different experiments. The ratio of mock sample was set = 1. (e) Representative image (20x magnification) of the immunofluorescence analysis showing MyHC expression at D3 of differentiation. DAPI staining was used to detect nuclei. (f) Fusion index relative to D3 calculated in at least five random microscopic fields.