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. 2017 Aug 3;7:7188. doi: 10.1038/s41598-017-07694-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Objective segmentation of the human sinus node and its paranodal area. This figure demonstrates high resolution (28 × 28 × 28 µm³) micro-CT data from part of the right atrium containing the sinus node. The extent of the sinus node and its paranodal area is outlined in short-axis (a–c) and long-axis (d) micro-CT images. (e) Volume rendering (endocardial view) showing objective segmentation of the low pixel values corresponding to the body of the sinus node in dark blue; pixel values corresponding to the paranodal area are shown in turquoise. The plane of section in a-c is shown on the 3D volume rendering (f). Arrows indicate the approximate seed point for the objective segmentation of the sinus node. Sinus node body length = 14.8mm, width = 4.3mm. The sections are available to view without the outlines in the supplementary Fig. S2. CT- terminal crest, SN- sinus node.