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. 2017 Aug 3;7:7252. doi: 10.1038/s41598-017-07354-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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16S rRNA gene diversity of Archaea in physicochemically and regionally distinct Yellowstone hot spring sediments. Taxonomic groupings of archaeal OTUs representing >0.1% of the sequences recovered from Washburn Hot Springs (WS) and Heart Lake Geyser Basin (HL). At one spring from each region—WS0 and HL9—a sediment push core was sectioned in three layers for separate microbial analysis, and the sediment depth in cm is labeled on each respective section of the circle chart with the deepest section closest to the center. Photographs of each hot spring are adjacent to diversity charts, and white circles indicate the location of sampling. Taxonomy is grouped by phylum and the next highest resolution taxonomic group possible is listed beneath the phylum as a separate color (c = class; o = order; f = family; g = genus). For Bathyarchaeota, MCG subgroups are listed according to classifications by Kubo and Lloyd et al. (2012) and Lazar et al. (2015), except for MCG-20, which is proposed in this study (Supplementary Figure S1). The map in this figure was obtained from the public domain national park maps available from the US National Park Service (https://www.nps.gov/hfc/carto/PDF/YELLmap2.pdf; licensing: https://www.nps.gov/hfc/carto/data-sources.cfm) and simplified with Adobe Illustrator CC 2015 (Adobe Systems; www.adobe.com/illustrator).