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. 2017 Aug 3;7:7179. doi: 10.1038/s41598-017-05840-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Scheme of cell-SELEX for GCGR-expressing CHO-K1 cells (CHO-GCGR). The ssDNA library was incubated with Mock cells as negative selection to remove the cell-binding ssDNA. The unbound ssDNA was incubated with GCGR-expressing CHO-K1 cells (CHO-GCGR) for positive selection. After washing, the bound DNA was eluted and amplified by PCR for next-round selection. The evolved ssDNA pool was sequenced to identify the aptamer candidates in the last round of selection.