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. 2017 Aug 3;7:7179. doi: 10.1038/s41598-017-05840-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Target location and type analysis of aptamer GR-3. (A) Binding site of aptamer GR-3 to CHO-GCGR cells was investigated by confocal microscopy imaging. (B) CHO-GCGR cells were treated with trypsin or proteinase K and then incubated with FAM-labeled GR-3 to analyze the target type by flow cytometry. (C) Identification of binding affinity between biotin-labeled GR-3 and GCGR protein by aptamer-mediated pull-down assay.