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. 2017 Aug 3;7:7211. doi: 10.1038/s41598-017-07830-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SLC39A4 silencing inhibits A549 cells metastasis in vitro. (a,b) SLC39A4 expression in A549 knockdown and empty vector control (Ctl) cells by western blot analysis (a) and qPCR (b,c). Zn²⁺ concentration in A549 cells after knockdown of SLC39A4 in vivo. (d,e) Analysis of SLC39A4 knockdown and Ctl cell migration in wound-healing assays (scale bar, 500 μm). Representative images (d) and quantitation (e) are shown. (f,g) Cell migration was monitored in transwell assays with SLC39A4 knockdown and Ctl A549 cells (scale bar, 100 μm). Representative images (f) and quantitation (g) are shown. (h,i) Cell invasion was monitored in matrigel transwell assays with SLC39A4 knockdown and Ctl A549 cells (scale bar, 100 μm). Representative images (h) and quantitation (i) are shown. (j,k) Analysis of E-cadherin (epithelial marker) and FSP-1 and N-cadherin (mesenchymal markers) expression in knockdown and control cells by immunofluorescence staining (j) and western blotting (k) (scale bar, 50 μm).