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. 2017 Aug 3;7:7255. doi: 10.1038/s41598-017-07267-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a,b) Photographs of the paper strips attached with tapes for the device fabrication. (c) The resulted 2D vPAD for colorimetric detection of four analytes (GLU: glucose, UA: uric acid, CHO: cholesterol, TG: triglycerides), scale bar: 2 mm. The colors of the paper strips are from the double-sided adhesive tapes used to assemble the device. (d) The resulted fluidic channels and flow direction (indicated by arrows) of aqueous sample in the device. (e) The vPAD for carrying out eight parallel assays. (f) The vPAD after introduction of 0.010 M coomassie brilliant blue solution. The assays results measured from the 2D vPAD for colorimetric detection of four analytes: (g) GLU: glucose; (h) UA: uric acid; (i) CHO: cholesterol; and (j) TG: triglycerides. The arrow bars indicate the standard deviation of three replicated measurements.