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. 2017 Aug 3;7:7261. doi: 10.1038/s41598-017-07391-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Induction of purified SimCells in PBS over time, as determined by GFP fluorescence per unit OD at 600 nm (n = 4). Error bars denote one standard deviation above and below the mean. (A) pCdaR SimCells induced by glucarate (significant induction relative to the control (p < 0.05) at 90 µM glucarate and above). (B) pAcuR SimCells induced by acrylate (significant induction at 5 mM acrylate and above). (C) pBAD SimCells induced by arabinose (significant induction at all concentrations tested). The OD 600 nm curves are shown in Fig. S5.