Table 9.
Method parameters and parameter values used during the PTA validation study
| Parameter | Level |
|---|---|
| Sample preparation—dilution factors | |
| PSL-46 | 1 × 105, 5 × 105 and 1 × 106 |
| PSL-100 | 1 × 105, 5 × 105 and 1 × 106 |
| ERM-FD102 | 1 × 103, 1.5 × 103 and 2 × 103 |
| Video acquisition | |
| Camera gain | 250–400 |
| Camera shutter | 12 ms to 25 ms |
| Frame rate | 25 frames/s |
| Capture time | 60 s |
| Video analysis | |
| Detection threshold | 4–10 |
| Blur | Automatic adjustment |
| Minimum expected particle size | Automatic adjustment |
| Minimum track length | Automatic adjustment |