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. 2017 Jul 26;284(1859):20170339. doi: 10.1098/rspb.2017.0339

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© 2017 The Author(s)

Published by the Royal Society. All rights reserved.

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Figure 1. — MDHns in macrolepidopteran moths innervate the ALs. (a) Schematic of MDHns (green) in M. sexta. Each MDHn innervates the SEZ and AMMC before projecting to both ALs. (b) Whole-mount M. sexta pterothoracic ganglion immunolabelled for HA. MDHns are the most anterior pair of histaminergic neurons (arrow heads). Depth: 166.6 µm. (c) Frontal section of M. sexta AL immunolabelled for HA (green). Bruchpilot immunolabelling delineates neuropil (magenta). Depth: 52.36 µm. (d) Sagittal section of B. mori MsN immunolabelled for HA. Depth: 133 µm. (e) Sagittal section of B. mori AL immunolabelled for HA (green). Bruchpilot immunolabelling delineates neuropil (magenta). Depth: 58.8 µm. (f) Whole-mount sagittal view of I. aemula pterothoracic ganglion. Depth: 164.64 µm. (g) Sagittal section of I. aemula AL immunolabelled for HA (green). Bruchpilot immunolabelling delineates neuropil (magenta). Bruchpilot (magenta) is used to delineate neuropil. HA-ir (green). Depth: 124 µm. Scale bars, 100 µm. Arrows, cell bodies; arrow heads, ascending axons; hatched white lines in (e) and (g) delineates the boundary of the AL.