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. 2017 Mar 3;66(3):199–208. doi: 10.1538/expanim.16-0097

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©2017 Japanese Association for Laboratory Animal Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 3. — Viral genome load in tissues of C57BL/6 mice infected with 10⁴ TCID₅₀ of BRC at 4, 8, 12, 16, 28, 56, 84, and 112 dpi. Tissues were blood (A), lungs (B), liver (C), spleen (D) and kidneys (E). The viral genome copies/ 500 ng RNA of each mouse tissue was calculated with the Thermal Cycler Dice Real Time System Software based on the respective standard curves for each virus strain (circles). Dashed line indicates detection limits of synthesized RNA determined by standard curves. Viral genome load of several samples exceeded detection limits of synthesized RNA determined by standard curves, and non-quantifiable samples were not indicated.